SpringerProtocols: Abstract: Bridging the Gene-to-Function Knowledge Gap Through Functional Genomics

9. Bridging the Gene-to-Function Knowledge Gap Through Functional Genomics

By: Stephen J. Robinson, Isobel A. P. Parkin⁴

Abstract

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The explosion of genomics data has led to a significant knowledge gap, with thousands of genes identified having no known function. The following chapter describes the available forward and reverse genetics strategies, which can assist researchers in assigning functions to novel genes. Details of the available resources for a number of model and crop species are provided. In addition, protocols are presented for utilising T-DNA tagged populations to identify genes underlying novel phenotypes and to assist with functional characterisation of target genes.

Affiliation(s): (4) Agriculture and Agri-Food, Saskatoon Research Centre, Saskatoon, Saskatchewan, S7N 0X2, Canada

Book Title: Plant Genomics: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 513 | Pub. Date: Mar-01-2008 | Page Range: 153-173 | DOI: 10.1007/978-1-59745-427-8_9

Subject: Plant Sciences

Key Words: Mutagenised population - Forward genetics - Reverse genetics - Phenotypic screening - Genetic variation

References

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