SpringerProtocols: Abstract: Transient Transformation of Plants

8. Transient Transformation of Plants

By: Huw D. Jones⁴, Angela Doherty, Caroline A. Sparks

Abstract

Full Text

Download PDF (302K)

Transient expression in plants is a valuable tool for many aspects of functional genomics and promoter testing. It can be used both to over-express and to silence candidate genes. It is also scaleable and provides a viable alternative to microbial fermentation and animal cell culture for the production of recombinant proteins. It does not depend on chromosomal integration of heterologous DNA so is a relatively facile procedure and can lead to high levels of transgene expression. Recombinant DNA can be introduced into plant cells via physical methods, via Agrobacterium or via viral vectors.

Affiliation(s): (4) CPI Division, Rothamsted Research, Harpenden, Hertfordshire, UK

Book Title: Plant Genomics: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 513 | Pub. Date: Mar-01-2008 | Page Range: 131-152 | DOI: 10.1007/978-1-59745-427-8_8

Subject: Plant Sciences

Key Words: Viral-induced gene silencing - Transgene - Gene delivery

References

Download References

1.	Abel, S. and Theologis, A. (1994) Transient transformation of Arabidopsis leaf protoplasts – a versatile experimental system to study gene-expression. Plant Journal 5, 421–427.

2.	Werr, W. and Lorz, H. (1986) Transient gene-expression in a Gramineae cell-line – a rapid procedure for studying plant promoters. Molecular and General Genetics 202, 471–475.

3.	Aoki, S. and Takebe, I. (1969) Infection of tobacco mesophyll protoplasts by tobacco mosaic virus ribonucleic acid. Virology 39, 439–448.

4.	Davey, M.R., Cocking, E.C., Freeman, J., Pearce, N., and Tudor, I. (1980) Transformation of Petunia protoplasts by isolated Agrobacterium plasmids. Plant Science Letters 18, 307–313.

5.	Sheen, J. (2001) Signal transduction in maize and Arabidopsis mesophyll protoplasts. Plant Physiology, 127, 1466-1475.

6.	Fromm, M.E., Morrish, F., Armstrong, C., Williams, R., Thomas, J., and Klein, T.M. (1990) Inheritance and expression of chimeric genes in the progeny of transgenic maize plants. Bio-Technology 8, 833–839.

7.	Klein, T.M., Fromm, M., Weissinger, A., Tomes, D., Schaaf, S., Sletten, M., and Sanford, J.C. (1988) Transfer of foreign genes into intact maize cells with high-velocity microprojectiles. Proceedings of the National Academy of Sciences of the United States of America 85, 4305–4309.

8.	Dekeyser, R.A., Claes, B., Derycke, R.M.U., Habets, M.E., Vanmontagu, M.C., and Caplan, A.B. (1990) Transient gene-expression in intact and organized rice tissues. Plant Cell 2, 591–602.

9.	Lindsey, K. and Jones, M.G.K. (1987) Transient gene-expression in electroporated protoplasts and intact-cells of sugar-beet. Plant Molecular Biology 10, 43–52.

10.	Nishiguchi, M., Langridge, W.H.R., Szalay, A.A., and Zaitlin, M. (1986) Electroporation-mediated infection of tobacco leaf protoplasts with tobacco mosaic-virus RNA and cucumber mosaic-virus RNA. Plant Cell Reports 5, 57–60.

11.	Krens, F.A., Molendijk, L., Wullems, G.J., and Schilperoort, R.A. (1982) Invitro transformation of plant-protoplasts with Ti-plasmid DNA. Nature 296, 72–74.

12.	Hillmer, S., Gilroy, S., and Jones, R.L. (1993) Visualizing enzyme-secretion from individual barley (Hordeum-Vulgare) Aleurone protoplasts. Plant Physiology 102, 279–286.

13.	Helenius, E., Boije, M., Niklander-Teeri, V., Palva, E.T., and Teeri, T.H. (2000) Gene delivery into intact plants using the Helios^TM Gene Gun. Plant Molecular Biology Reporter 18, 287–288.

14.	Hellens, R., Allan, A., Friel, E., Bolitho, K., Grafton, K., Templeton, M., Karunairetnam, S., Gleave, A., and Laing, W. (2005) Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants. Plant Methods 1, 446–451.

15.	Gheysen, G., Angenon, G., and Van Montagu, M. (1998) Agrobacterium-mediated plant transformation: a scientifically intriguing story with significant applications, in Transgenic Plant Research (Lindsey, K., ed.), Harwood Academic Press, the Netherlands, pp. 1–33.

16.	Kapila, J., DeRycke, R., VanMontagu, M., and Angenon, G. (1997) An Agrobacterium-mediated transient gene expression system for intact leaves. Plant Science 122, 101–108.

17.	Joh, L.D., Wroblewski, T., Ewing, N.N., and VanderGheynst, J.S. (2005) High-level transient expression of recombinant protein in lettuce. Biotechnology and Bioengineering 91, 861–871.

18.	Scholthof, H.B., Scholthof, K.B.G., and Jackson, A.O. (1996) Plant virus gene vectors for transient expression of foreign proteins in plants. Annual Review of Phytopathology 34, 299–323.

19.	Streatfield, S.J. (2007) Approaches to achieve high-level heterologous protein production in plants. Plant Biotechnology Journal 5, 2–15.

20.	Gelvin, S.B. (2005) Viral-mediated plant transformation gets a boost. Nature Biotechnology 23, 684–685.

21.	Negrouk, V., Eisner, G., Lee, H.I., Han, K.P., Taylor, D., and Wong, H.C. (2005) Highly efficient transient expression of functional recombinant antibodies in lettuce. Plant Science 169, 433–438.

22.	Gleba, Y., Marillonnet, S., and Klimyuk, V. (2004) Engineering viral expression vectors for plants: the ‘full virus’ and the ‘deconstructed virus’ strategies. Current Opinion in Plant Biology 7, 182–188.

23.	Marillonnet, S., Thoeringer, C., Kandzia, R., Klimyuk, V., and Gleba, Y. (2005) Systemic Agrobacterium tumefaciens-mediated transfection of viral replicons for efficient transient expression in plants. Nature Biotechnology 23, 718–723.

24.	Gleba, Y., Klimyuk, V., and Marillonnet, S. (2005) Magnifection-a new platform for expressing recombinant vaccines in plants. Vaccine 23, 2042–2048.

25.	Baulcombe, D.C. (1999) Gene silencing-RNA makes RNA makes no protein. Current Biology 9, R599–R601.

26.	Lindbo, J.A., Silvarosales, L., Proebsting, W.M., and Dougherty, W.G. (1993) Induction of a highly specific antiviral state in transgenic plants – implications for regulation of gene-expression and virus-resistance. Plant Cell 5, 1749–1759.

27.	Watson, J.M., Fusaro, A.F., Wang, M.B., and Waterhouse, P.M. (2005) RNA silencing platforms in plants. FEBS Letters 579, 5982–5987.

28.	Jin, H.L., Axtell, M.J., Dahlbeck, D., Ekwenna, O., Zhang, S.Q., Staskawicz, B., and Baker, B. (2002) NPK1, an MEKK1-like mitogen-activated protein kinase kinase kinase, regulates innate immunity and development in plants. Developmental Cell 3, 291–297.

29.	Thomas, C.L., Jones, L., Baulcombe, D.C., and Maule, A.J. (2001) Size constraints for targeting post-transcriptional gene silencing and for RNA-directed methylation in Nicotiana benthamiana using a potato virus X vector. Plant Journal 25, 417–425.

30.	Burch-Smith, T.M., Anderson, J.C., Martin, G.B., and Dinesh-Kumar, S.P. (2004) Applications and advantages of virus-induced gene silencing for gene function studies in plants. Plant Journal 39, 734–746.

31.	Ratcliff, F., Martin-Hernandez, A.M., and Baulcombe, D.C. (2001) Tobacco rattle virus as a vector for analysis of gene function by silencing. Plant Journal 25, 237–245.

32.	Burch-Smith, T.M., Schiff, M., Liu, Y.L., and Dinesh-Kumar, S.P. (2006) Efficient virus-induced gene silencing in Arabidopsis. Plant Physiology 142, 21–27.

33.	Robertson, D. (2004) VIGS vectors for gene silencing: many targets, many tools. Annual Review of Plant Biology 55, 495–519.

34.	Fromm, M., Callis, J., Taylor, L.P., and Walbot, V. (1987) Electroporation of DNA and RNA into plant-protoplasts. Methods in Enzymology 153, 351–366.

35.	Hauptmann, R.M., Oziasakins, P., Vasil, V., Tabaeizadeh, Z., Rogers, S.G., Horsch, R.B., Vasil, I.K., and Fraley, R.T. (1987) Transient expression of electroporated DNA in monocotyledonous and dicotyledonous species. Plant Cell Reports 6, 265–270.

36.	Wang, Y.C., Klein, T.M., Fromm, M., Cao, J., Sanford, J.C., and Wu, R. (1988) Transient expression of foreign genes in Rice, Wheat and Soybean cells following particle bombardment. Plant Molecular Biology 11, 433–439.

37.	Bhattacharyya, S., Dey, N., and Maiti, I.B. (2002) Analysis of cis-sequence of subgenomic transcript promoter from the Figwort mosaic virus and comparison of promoter activity with the cauliflower mosaic virus promoters in monocot and dicot cells. Virus Research 90, 47–62.

38.	Blechl, A.E., Lorens, G.F., Greene, F.C., Mackey, B.E., and Anderson, O.D., (1994) A transient assay for promoter activity of wheat seed storage protein genes and other genes expressed in developing endosperm. Plant Science 102, 69–80.

39.	He, X.Y., Futterer, J., and Hohn, T. (2002) Contribution of downstream promoter elements to transcriptional regulation of the rice tungro bacilliform virus promoter. Nucleic Acids Research 30, 497–506.

40.	Martinez, A., Sparks, C., Hart, C.A., Thompson, J., and Jepson, I. (1999) Ecdysone agonist inducible transcription in transgenic tobacco plants. Plant Journal 19, 97–106.

41.	Martinez, A., Sparks, C., Drayton, P., Thompson, J., Greenland, A., and Jepson, I. (1999) Creation of ecdysone receptor chimeras in plants for controlled regulation of gene expression. Molecular and General Genetics 261, 546–552.

42.	Sun, C.X., Palmqvist, S., Olsson, H., Boren, M., Ahlandsberg, S., and Jansson, C. (2003) A novel WRKY transcription factor, SUSIBA2, participates in sugar signaling in barley by binding to the sugar-responsive elements of the iso1 promoter. Plant Cell 15, 2076–2092.

43.	Vickers, C.E., Xue, G.P., and Gresshoff, P.M. (2006) A novel cis-acting element, ESP, contributes to high-level endosperm-specific expression in an oat globulin promoter. Plant Molecular Biology 62, 195–214.

44.	Sivamani, E. and Qu, R. (2006) Expression enhancement of a rice polyubiquitin gene promoter. Plant Molecular Biology 60, 225–239.

45.	Yoshida, K. and Shinmyo, A. (2000) Transgene expression systems in plant, a natural bioreactor. Journal of Bioscience and Bioengineering 90, 353–362.

46.	Ma, J.K.C., Drake, P.M.W., Chargelegue, D., Obregon, P., and Prada, A. (2005) Antibody processing and engineering in plants, and new strategies for vaccine production. Vaccine 23, 1814–1818.

47.	Giritch, A., Marillonnet, S., Engler, C., van Eldik, G., Botterman, J., Klimyuk, V., and Gleba, Y. (2006) Rapid high-yield expression of full-size IgG antibodies in plants coinfected with noncompeting viral vectors. Proceedings of the National Academy of Sciences of the United States of America 103, 14701–14706.

48.	Sudarshana, M.R., Plesha, M.A., Uratsu, S.L., Falk, B.W., Dandekar, A.M., Huang, T.K., and McDonald, K.A. (2006) A chemically inducible cucumber mosaic virus amplicon system for expression of heterologous proteins in plant tissues. Plant Biotechnology Journal 4, 551–559.

49.	Garfinkel, D.J. and Nester, E.W. (1980) Agrobacterium-Tumefaciens Mutants Affected in Crown Gall Tumorigenesis and Octopine Catabolism. Journal of Bacteriology, 144, 732-743.

50.	Jefferson, R.A., Kavanagh, T.A., and Bevan, M.W. (1987) GUS fusion: β-Glucuronidase as a sensitive and versatile gene fusion marker in plants. EMBO J. 6, 3901–3907.

51.	Rasco-Gaunt, S., Riley, A., Barcelo, P., and Lazzeri, P.A. (1999) Analysis of particle bombardment parameters to optimise DNA delivery into wheat tissues. Plant Cell Reports 19, 118–127.

Comments (Loading...)

Post comment/View all comments