SpringerProtocols: Abstract: Stable Transformation of Plants

7. Stable Transformation of Plants

By: Huw D. Jones⁴

, Caroline A. Sparks

Abstract

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This chapter provides an overview of the main steps in the process to produce stably transformed plants. Most transformation methods use tissue culture to recover adult plants from regenerable explants and can be divided into three stages: (1) choice and preparation of explant tissue, (2) deoxyribonucleic acid (DNA) delivery, (3) callus induction/regeneration and selection. Each of these stages is introduced from a general perspective and a detailed protocol for our exemplar species, wheat, is given. We focus here on DNA delivery by particle bombardment as Agrobacterium-mediated transformation methods for wheat are reported elsewhere (29).

Affiliation(s): (4) CPI Division, Rothamsted Research, Harpenden, Hertfordshire, UK

Book Title: Plant Genomics: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 513 | Pub. Date: Mar-01-2008 | Page Range: 111-130 | DOI: 10.1007/978-1-59745-427-8_7

Subject: Plant Sciences

Key Words: Transformation - Particle bombardment - Explant - Tissue culture - Selection - DNA delivery - Transgene - Wheat

References

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