Springer Protocols: Abstract: Applications of Functional Protein Microarrays: Identifying Protein-Protein Interactions in an Array Format

Applications of Functional Protein Microarrays: Identifying Protein-Protein Interactions in an Array Format

By: Matthew A. Coleman², Peter T. Beernink², Julio A. Camarero³, Joanna S. Albala⁴

Abstract

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The use of protein arrays and their importance in proteomic applications continues to be at the forefront of scientific discovery and innovative technology development. To date, array-based approaches have proven to be a powerful tool for protein expression profiling, novel biomarker discovery, and the examination of protein, DNA, and small molecule interactions. Our laboratory has developed several approaches for characterizing protein-protein interactions using protein microarrays for a variety of different biological applications. Here we describe the identification of protein-protein interactions using a microarray format.

Affiliation(s): (2) Biosciences Division, Lawrence Livermore National Laboratory, Livermore, CA
(3) Chemical and Materials Sciences Division, Lawrence Livermore National Laboratory, Livermore, CA
(4) Department of Otolaryngology, University of California at Davis, Sacramento, CA

Book Title: Microchip-Based Assay Systems: Methods and Applications

Series: Methods in Molecular Biology | Volume: 385 | Pub. Date: Nov-13-2007 | Page Range: 121-130 | DOI: 10.1007/978-1-59745-426-1_9

Subject: Genetics/Genomics

Key Words: Protein microarray - in vitro expression - fluorescence - green fluorescent protein - protein interactions

References

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