SpringerProtocols: Abstract: A Microchip-Based Assay for Interleukin-6

A Microchip-Based Assay for Interleukin-6

By: Nicolaos Christodoulides², Priya Dharshan², Jorge Wong², Pierre N. Floriano², Dean Neikirk², John T. McDevitt²

Abstract

Full Text

Download PDF (229K)

The electronic taste chip (ETC) assay system is a lab-on-a-chip technology that offers a microchip platform on which bead-based immunoassays are performed. Each bead within the array serves as its own independent self-contained “microreactor” system, with its selectivity determined by the specificity of the antibody that it hosts. The bead-loaded chip is sandwiched between two optically transparent polymethylmethacrylate inserts, packaged within a metal casing described here as the “flow cell.” This flow cell allows for delivery of sample and detecting reagents to the microchip and the associated beads. Images of fluorescent beads are captured with a digital video chip and analyzed to facilitate detection and, ultimately, quantitation of analytes in complex fluids. This chapter describes the application of the ETC system for the detection and measurement of interleukin (IL)-6.

Affiliation(s): (2) Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, TX

Book Title: Microchip-Based Assay Systems: Methods and Applications

Series: Methods in Molecular Biology | Volume: 385 | Pub. Date: Nov-13-2007 | Page Range: 131-144 | DOI: 10.1007/978-1-59745-426-1_10

Subject: Genetics/Genomics

Key Words: Coronary heart disease - interleukin-6 - electronic taste chip - bead-based immunoassay - lab on a chip - inflammation

References

Download References

1.	Papanicolaou, D. A., Wilder, R. L., Manolagas, S. C., and Chrousos, G. P. (1998) The pathophysiologic roles of interleukin-6 in human disease. Ann. Intern. Med. 128, 127–137.

2.	Baumann, H. and Gauldie, J. (1990) Regulation of hepatic acute phase plasma-protein genes by hepatocyte stimulating factors and other mediators of inflammation. Mol. Biol. Med. 7, 147–159.

3.	Heinrich, P. C., Castell, J. V., and Andus, T. (1990) Interleukin-6 and the acute phase response. Biochem. J. 265, 621–636.

4.	Ridker, P. M., Buring, J. E., Shih, J., Matias, M., and Hennekens, C. H. (1998) Prospective study of C-reactive protein and the risk of future cardiovascular events among apparently healthy women. Circulation 98, 731–733.

5.	Ridker, P. M., Cushman, M., Stampfer, M. J., Tracy, R. P., and Hennekens, C. H. (1997) Inflammation, aspirin, and the risk of cardiovascular disease in apparently healthy men. N. Engl. J. Med. 336, 973–979.

6.	Ridker, P. M., Rifai, N., Stampfer, M. J., and Hennekens, C. H. (2000) Plasma concentration of interleukin-6 and the risk of future myocardial infarction among apparently healthy men. Circulation 101, 1767–1772.

7.	Bauer, J., Ganter, U., Strauss, S., et al. (1992) The participation of interleukin-6 in the pathogenesis of Alzheimers disease. Res. Immunol. 143, 650–656.

8.	Borg, S. A., Kerry, K. E., Royds, J. A., Battersby, R. D., and Jones, T. H. (2005) Correlation of VEGF production with IL1α and IL6 secretion by human pituitary adenoma cells. Eur. J. Endocrinol. 152, 293–300.

9.	Filion, L. G., Graziani-Bowering, G., Matusevicius, D., and Freedman, M. S. (2003) Monocyte-derived cytokines in multiple sclerosis. Clin. Exp. Immunol. 131, 324–334.

10.	Nagatsu, T. and Sawada, M. (2005) Inflammatory process in Parkinson. Curr. Pharm. Design 11, 999–1016.

11.	Waje-Andreassen, U., Krakenes, J., Ulvestad, E., et al. (2005) IL-6: an early marker for outcome in acute ischemic stroke. Acta Neurol. Scand. 111, 360–365.

12.	Maier, B., Laurer, H. L., Rose, S., Buurman, W. A., and Marzi, I. (2005) Physiological levels of pro-and anti-inflammatory mediators in cerebrospinal fluid and plasma: a normative study. J. Neurotrauma 22, 822–835.

13.	Penkowa, M., Giralt, M., Lago, N., et al. (2003) Astrocyte-targeted expression of IL-6 protects the CNS against a focal brain injury. Exp. Neurol. 181, 130–148.

14.	Goodey, A., Lavigne, J. J., Savoy, S. M., et al. (2001) Development of multianalyte sensor arrays composed of chemically derivatized polymeric microspheres localized in micromachined cavities. J. Am. Chem. Soc. 123, 2559–2570.

15.	Lavigne, J. J., Savoy, S., Clevenger, M. B., et al. (1998) Solution-based analysis of multiple analytes by a sensor array: Toward the development of an “electronic tongue.” J. Am. Chem. Soc. 120, 6429–6430.

16.	Gustavsson, P.-E., Axelsson, A., and Larsson, P.-O. (1999) Superporous agarose beads as a hydrophobic interaction chromatography support. J. Chromatography A 830, 275–284.

17.	Gustavsson, P.-E. and Larsson, P.-O. (1996) Superporous agarose, a new material for chromatography. J. Chromatography A 734, 231–240.

18.	Gustavsson, P.-E., Mosbach, K., Nilsson, K., and Larsson, P.-O. (1997) Superporous agarose as an affinity chromatography support. J. Chromatography A 776, 197–203.

Comments (Loading...)

Post comment/View all comments