SpringerProtocols: Abstract: 8. Physcomitrella patens : A Non-Vascular Plant for Recombinant Protein Production

8. Physcomitrella patens : A Non-Vascular Plant for Recombinant Protein Production

By: David Liénard³, Fabien Nogué⁴

Abstract

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The moss Physcomitrella patens is a long-standing model for studying plant development, growth and cell differentiation in particular. Interest in this non-vascular plant arose following the discovery that homologous recombination is an efficient process. P. patens is, therefore, a tool of choice not only to study gene function but also for recombinant protein production. This system has many attributes that are advantageous for molecular farming: protein production in cell suspension, the possibility of generating targeted knockout mutants for glycoengineering and quantitative optimization for protein production. In terms of technical advances, P. patens is one of the most up-to-date plant expression systems and is a promising alternative to animal cell factories for the production of therapeutic proteins with either simple or highly complex structures.

Affiliation(s): (3) Faculté des Sciences, Université de Rouen, Mont Saint Aignan, France
(4) Station de Génétique et Amélioration des plantes, INRA, Route de St Cyr, Versailles, France

Book Title: Recombinant Proteins From Plants: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 483 | Pub. Date: Jun-01-2008 | Page Range: 135-144 | DOI: 10.1007/978-1-59745-407-0_8

Subject: Plant Sciences

Key Words: Physcomitrella Patens - Moss - Homologous recombination - Cre/lox - Recombinant protein production

References

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