SpringerProtocols: Abstract: Nuclear Magnetic Resonance Methods for Monitoring Cell Growth and Metabolism in Intensive Bioreactors

Nuclear Magnetic Resonance Methods for Monitoring Cell Growth and Metabolism in Intensive Bioreactors

By: André A. Neves², Kevin M. Brindle²

Abstract

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Magnetic resonance imaging (MRI) and spectroscopy (MRS) are powerful noninvasive techniques that can be used to monitor the behavior of cells in intensive bioreactor systems. We describe here a number of nuclear magnetic resonance (NMR)-based techniques that have been used successfully to investigate cell growth and distribution, cellular energetics and the porosity to medium flow and linear flow velocity profiles around and across cell layers in perfusion bioreactors. These are important parameters to determine when designing bioreactor systems and operation protocols that optimize cell productivity.

Affiliation(s): (2) Biochemistry Department, University of Cambridge, Cambridge, UK

Book Title: Animal Cell Biotechnology: Methods and Protocols

Series: Methods in Biotechnology | Volume: 24 | Pub. Date: Apr-05-2007 | Page Range: 239-251 | DOI: 10.1007/978-1-59745-399-8_10

Subject: Biotechnology

Key Words: Nuclear magnetic resonance - magnetic resonance imaging - magnetic resonance spetroscopy - cell growth - cell metabolism - bioreactors

References

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