Springer Protocols: Abstract: Incorporation of Gene Ontology Annotations to Enhance Microarray Data Analysis

Incorporation of Gene Ontology Annotations to Enhance Microarray Data Analysis

By: Michael F. Ochs², Aidan J. Peterson², Andrew Kossenkov², Ghislain Bidaut³

Abstract

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Typical microarray or GeneChip™ experiments now provide genome-wide measurements on gene expression across many conditions. Analysis often focuses on only a few of the genes, looking for those that are “differentially expressed” between conditions or groups of conditions. However, the large number of measurements both present statistical problems to such single gene approaches and offers a tremendous amount of information for methods focused on biological processes rather than individual genes. Here we provide a method to utilize biological annotations in the form of gene ontologies to interpret the results of individual or multiple pattern recognition analyses of a microarray experiment.

Affiliation(s): (2) Fox Chase Cancer Center, Philadelphia, PA
(3) Center for Bioinformatics, Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia, PA

Book Title: Microarray Data Analysis: Methods and Applications

Series: Methods in Molecular Biology | Volume: 377 | Pub. Date: May-03-2007 | Page Range: 243-254 | DOI: 10.1007/978-1-59745-390-5_15

Subject: Genetics/Genomics

Key Words: Microarray - gene ontology - biological process - pattern recognition - clustering

References

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