Springer Protocols: Abstract: Methods for High-Throughput Materialization of Genetic Information Based on Wheat Germ Cell-Free Expression System

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Methods for High-Throughput Materialization of Genetic Information Based on Wheat Germ Cell-Free Expression System

By: Tatsuya Sawasaki², Ryo Morishita³, Mudeppa D. Gouda², Yaeta Endo²

Abstract

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Among the cell-free protein synthesis systems, the wheat germ-based translation system has significant advantages for the high-throughput production of eukaryotic multidomain proteins in folded state. Here, we describe protocols for this cell-free expression system.

Affiliation(s): (2) Cell-Free Science and Technology Research Center, Ehime University, Matsuyama, Japan
(3) Cell-Free Science, Co., Ltd., Kanagawa, Japan

Book Title: In Vitro Transcription and Translation Protocols

Series: Methods in Molecular Biology | Volume: 375 | Pub. Date: May-03-2007 | Page Range: 95-106 | DOI: 10.1007/978-1-59745-388-2_5

Subject: Genetics/Genomics

Key Words: Cell-free protein synthesis - wheat germ extract - 5′- and 3′-UTRs - PCR - using split-primers - transcription and translation - purification of products

References

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