SpringerProtocols: Abstract: 7. Practical Considerations for the Analysis of Ionic and Neutral Organic Molecules With Capillary Electrophoresis/Mass Spectrometry

7. Practical Considerations for the Analysis of Ionic and Neutral Organic Molecules With Capillary Electrophoresis/Mass Spectrometry

By: Moritz Frommberger³, Matthias Englmann³, Philippe Schmitt Kopplin³

Abstract

Full Text

Download PDF (897K)

This chapter presents the technique of capillary electrophoresis coupled to mass spectrometry (CE/MS). The introductory section is targeted mainly at CE/MS beginners and notes briefly the theoretical background of electrospray ionization (ESI), the most commonly used ionization mode in CE/MS. The specifics of CE/MS are described—also in comparison with more classic methods like LC/MS. Important caveats to be taken nto consideration for successful CE/MS operation are noted in the interest of avoiding pitfalls. CE/MS is illustrated with three representative examples, which might serve as starting points for more in-detail experiments: (1) partial-filling micellar elektrokinetic chromatography (MEKC) ofneutral bacterial signaling molecules (N-acylhomoserine lactones) extracted from culture supernatants, (2) capillary zone electrophoresis (CZE) of their anionic degradation products, and finally (3) CZE separation of cationic hydroxys-triazines.

Affiliation(s): (3) HelmholtzZentrum München, German Research Center for Environmental Health, Institute of Ecological Chemistry/Chemical BioGeoAnalysis, BioGeomics, Neuherberg, Germany

Book Title: Capillary Electrophoresis: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 384 | Pub. Date: Jan-04-2008 | Page Range: 135-156 | DOI: 10.1007/978-1-59745-376-9_7

Subject: Cell Biology

Key Words: Capillary electrophoresis - mass spectrometry - electrospray ionization - coupling techniques - anions - cations - neutrals - N-acylhomoserine lactones - triazines

References

Download References

1.	Olivares, J. A., Nguyen, N. T., Yonker, C. R., and Smith, R. D. (1987) On-line mass spectrometric detection for CZE. Anal. Chem. 59, 1230–1232.

2.	Schmitt-Kopplin, P. and Frommberger, M. (2003) Capillary electrophoresis mass spectrometry: 15 years of developments and applications. Electrophoresis 24, 3837–3867.

3.	Schmitt-Kopplin, P. and Englmann, M. (2005) Capillary electrophoresis mass spectrometry: Survey on developments and applications 2003–2004. Electrophoresis 26, 1209–1220.

4.	Fenn, J. B. (2003) Electrospray wings for molecular elephants (Nobel lecture). Angew. Chem. Int. Edit. 42, 3871–3894.

5.	Caprioli, R. and Gross, M. (2005) The Encyclopedia of Mass Spectrometry. Volume 8, Hyphenated Methods. Elsevier, Amsterdam.

6.	Abian, J., Oosterkamp, A. J., and Gelpi, E. (1999) Comparison of conventional, narrow-bore and capillary liquid chromatography mass spectrometry for electrospray ionization mass spectrometry: practical considerations. J. Mass Spectrom. 34, 244–254.

7.	Kebarle, P. and Tang, L. (1993) From ions in solutions to ions in the gas phase: the mechanism of electrospray mass spectrometry. Anal. Chem. 65, 972–986.

8.	Rundlett, K. L. and Armstrong, D. W. (1996) Mechanism of signal suppression by anionic surfactants in capillary electrophoresis electrospray ionization mass spectrometry. Anal. Chem. 68, 3493–3498.

9.	Yang, L. and Lee, C. S. (1997) Micellar electrokinetic chromatography-mass spectrometry. J. Chromatogr. A 780, 207–218.

10.	Cole, R. B. (2000) Some tenets pertaining to electrospray ionization mass spectrometry. J. Mass Spectrom. 35, 763–772.

11.	Fernandez de la Mora, J., van Berkel, G. J., Enke, C. G., Cole, R. B., Martinez-Sanchez, M., and Fenn, J. B. (2000) Electrochemical processes in electrospray ionization mass spectrometry. J. Mass Spectrom. 35, 939–952.

12.	Agilent Technologies (1999) CE-ESI-MS: an integrated solution. Publication Number 5968-1328E.

13.	Frommberger, M., Schmitt-Kopplin, P., Menzinger, F., et al. (2003) Analysis of N-acyl-textscl-homoserine lactones produced by Burkholderia cepacia with partial filling micellar electrokinetic chromatography: electrospray ionization-ion trap mass spectrometry. Electrophoresis 24, 3067–3074.

14.	Fuqua, C. and Greenberg, E. (2002) Listening in on bacteria: acyl-homoserine lactone signalling. Nat. Rev. Mol. Cell Biol. 3, 685–695.

15.	Messina, P., Morini, M. A., Schulz, P. C., and Ferrat, G. (2002) The aggregation of sodium dehydrocholate in water. Colloid Polym. Sci. 280, 328.

16.	Molina, M., Wiedmer, S. K., Jussila, M., Silva, M., and Riekkola, M. L. (2001) Use of a partial filling technique and reverse migrating micelles in the study of N-methylcarbamate pesticides by micellar electrokinetic chromatography-electrospray ionization mass spectrometry. J. Chromatogr. A 927, 191–202.

17.	Frommberger, M., Hertkorn, N., Englmann, M., et al. (2005) Analysis of N-acylhomoserine lactones after alkaline hydrolysis and anion-exchange solid-phase extraction by capillary zone electrophoresis-mass spectrometry. Electrophoresis 26, 1523–1532.

18.	Schmitt, P., Poiger, T., Simon, R., Freitag, D., Kettrup, A., and Garrison, A. W. (1997) Simultaneous determination of ionization constants and isoelectric points of 12 hydroxy-s-triazines by capillary zone electrophoresis and capillary isoelectric focusing. Anal. Chem. 69, 2559–2566.

Comments (Loading...)

Post comment/View all comments