SpringerProtocols: Abstract: 12. Capillary Electrophoresis of Sugar Acids

12. Capillary Electrophoresis of Sugar Acids

By: Cristiana Campa³, Edi Baiutti⁴, Anna Flamigni³

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This chapter illustrates the usefulness of capillary electrophoresis (CE) for the analysis of sugar acids, that is, monosaccharides and lower oligosaccharides carrying carboxylate, sulphate or phosphate groups. In order to provide a general description of the main results and challenges in the field, some relevant applications and reviews on CE of such saccharidic compounds are tabulated. Furthermore, some detailed experimental procedures are shown, regarding the CE analysis of sugar acids released upon hydrolysis of acidic polysaccharides and of glycans linked to glycoproteins. In particular, the protocols will deal with the following compounds: (i) unsaturated, underivatized oligosaccharides from lyase-treated alginate; (ii) oligosaccharides derivatized with 4-aminobenzonitrile, arising from chemical hydrolysis of alginate; (iii) sialic acid derivatized with 2-aminoacridone, released from human serum immunoglobulin G.

Affiliation(s): (3) Novartis Vaccines and Diagnostics, Siena, Italy
(4) Bracco Imaging SpA-CRM Trieste, AREA Science Park, Trieste, Italy

Book Title: Capillary Electrophoresis: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 384 | Pub. Date: Jan-04-2008 | Page Range: 307-355 | DOI: 10.1007/978-1-59745-376-9_12

Subject: Cell Biology

Key Words: Capillary electrophoresis - sugar acids - sugar phosphates - carboxylated sugars - sulphated sugars - glycosaminoglycans oligosaccharides - uronic acids - sialic acids - tabulated review

References

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