SpringerProtocols: Abstract: 10. Capillary Electrophoresis for Pharmaceutical Analysis

10. Capillary Electrophoresis for Pharmaceutical Analysis

By: Alex Marsh³, Margo Broderick⁴, Kevin Altria³, Joe Power⁴, Sheila Donegan³, Brian Clark⁵

Abstract

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This chapter describes the application of capillary electrophoresis (CE) to pharmaceutical analysis. The areas of pharmaceutical analysis covered are enantiomer separation, analysis of small molecules such as amino acids or drug counter-ions, pharmaceutical assay, related substances determinations, and physiocochemical measurements such as log P and pKa of compounds. The different electrophoretic modes available and their advantages for pharmaceutical analysis are described. Recent applications of CE for each subject area are tabulated with electrolyte details. Information on electrolyte choice and method optimization to obtain optimal separations is included.

Affiliation(s): (3) GlaxoSmithKline Research & Development, Harlow, Essex, UK
(4) Waterford Institute of Technology, Department of Chemical and Life Sciences, Waterford, Ireland
(5) Univeristy of Bradford, School of Pharmacy, Bradford, UK

Book Title: Capillary Electrophoresis: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 384 | Pub. Date: Jan-04-2008 | Page Range: 205-245 | DOI: 10.1007/978-1-59745-376-9_10

Subject: Cell Biology

Key Words: Capillary electrophoresis - free solution CE - nonaqueous CE - micellar electrokinetic chromatography - microemulsion electrokinetic chromatography - pharmaceuticals - review - dynamic capillary coating - chiral separation - log P determination - pKa determination - assay

References

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