SpringerProtocols: Abstract: Protein Stabilization by the Rational Design of Surface Charge?Charge Interactions

11. Protein Stabilization by the Rational Design of Surface Charge–Charge Interactions

By: Katrina L. Schweiker², George I. Makhatadze²

Abstract

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The design of proteins with increased stability has many important applications in biotechnology. In recent years, strategies involving directed evolution, sequence-based design, or computational design have proven successful for generating stabilized proteins. A brief overview of the various methods that have been used to increase protein stability is presented, followed by a detailed example of how the rational design of surface charge–charge interactions has provided a robust method for protein stabilization.

Affiliation(s): (2) Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, USA

Book Title: Protein Structure, Stability, and Interactions

Series: Methods in Molecular Biology | Volume: 490 | Pub. Date: Mar-01-2008 | Page Range: 1-23 | DOI: 10.1007/978-1-59745-367-7_11

Subject: Protein Science

Key Words: Protein stability - computational design - surface charge–charge interactions

References

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