SpringerProtocols: Abstract: 9. Monocyte-Derived Dendritic Cells as Antigen-Presenting Cells in T-Cell Proliferation and Cytokine Production

9. Monocyte-Derived Dendritic Cells as Antigen-Presenting Cells in T-Cell Proliferation and Cytokine Production

By: Sun-sang J. Sung³

Abstract

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Dendritic cells (DC) are widely considered to be the major antigen-presenting cell (APC) type in immune responses. These cells are obtained from adherent cells or are purified CD14⁺ monocytes from peripheral blood mononuclear cells (PBMC) by in vitro stimulation with granulocyte, macrophage-colony-stimulating factor (GM-CSF) plus interleukin (IL)-4. They express high levels of MHC class II and costimulatory molecules, internalize Ag rapidly via Fc receptors and mannose receptors, and, by macropinocytosis, produce large amounts of IL-12 on CD40 ligation, and are potent in presenting soluble Ag and in stimulating allogeneic mixed-leukocyte reactions.

To study primary T-cell responses and cytokine production in allergy patients, we have developed an in vitro system by using highly purified T cells as responder cells and monocyte-derived DC (MDC) as the APC. MDC provide a convenient and potent APC source for T-cell response studies.

Affiliation(s): (3) Division of Rheumatology, University of Virginia Health Sciences Center, Charlottesville, VA

Book Title: Allergy Methods and Protocols

Series: Methods in Molecular Medicine | Volume: 138 | Pub. Date: Dec-07-2007 | Page Range: 97-106 | DOI: 10.1007/978-1-59745-366-0_9

Subject: Microbiology

Key Words: Antigen-presenting cells - dendritic cells - T-cell proliferation - cytokines

References

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