SpringerProtocols: Abstract: Laser Capture Microdissection of Kidney Tissue

6. Laser Capture Microdissection of Kidney Tissue

By: Robert P. Woroniecki³, Erwin P. Bottinger⁴

Abstract

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Kidney tissue laser capture microdissection (LCM) is of great clinical relevance since genome wide studies on total kidney messenger RNA (mRNA) potentially miss important factors involved in the pathogenesis of the disease in glomeruli and tubules. This technique is readily applicable to study mRNA from isolated glomeruli and tubules of human kidney biopsy material. In this chapter we present a “cook-book” practical approach of utilizing LCM in combination with RNA isolation technique in downstream applications in nephrology.

Affiliation(s): (3) Department of Pediatrics, The Children’s Hospital at Montefiore, Albert Einstein College of Medicine, New York, NY, USA
(4) Department of Medicine, Mount Sinai School of Medicine, New York, NY, USA

Book Title: Kidney Research: Experimental Protocols

Series: Methods in Molecular Biology | Volume: 466 | Pub. Date: Jul-01-2008 | Page Range: 1-10 | DOI: 10.1007/978-1-59745-352-3_6

Subject: Molecular Medicine

Key Words: Glomerular isolation - Tubular dissection - RNA quality and quantity

References

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