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Assessing Expression of Apoptotic Markers Using Large Cohort Tissue Microarrays
Abstract
Apoptotic markers include proteins from the intrinsic and extrinsic pathways. These cascades include both pro-apoptotic and anti-apoptotic elements. The expression levels of these elements can be assessed by immunohistochemistry (IHC) and can indicate general trends in pro- versus anti-apoptotic tendencies of the cells. IHC is particularly useful when studying large cohorts of paraffin-embedded specimens. Advances in tissue microarray (TMA) technology have facilitated evaluation of large cohorts of specimens, as cores from hundreds of patients can be represented on a single glass slide and stained in a uniform fashion. In this chapter, we discuss construction and staining methods of TMAs and present examples of assessment of apoptotic marker expression in malignant and benign cells using a novel method of automated, quantitative analysis of in situ protein expression.
Affiliation(s): (2) Department of Medicine, Yale University School of Medicine, New Haven, CT
Series: Methods in Molecular Biology  |  Volume: 414  |  Pub. Date: Oct-11-2007  |  Page Range: 83-93  |  DOI: 10.1007/978-1-59745-339-4_8
Subject:  Cancer Research
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