SpringerProtocols: Abstract: The Application of Differential Display as a Gene Profiling Tool

The Application of Differential Display as a Gene Profiling Tool

By: Ken Chien-Neng Chang⁴, Barry Komm⁴, Nichole Bayer Arnold⁵, Murray Korc^{5 6}

Abstract

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Differential display is an effective expression profiling tool which was first introduced in 1992. The original technique is discussed along with modifications that have been described over the last several years. A highly reproducible, semihigh-throughput differential display protocol used in our laboratories is described along with an example of its successful application using pancreatic cancer cells. In addition to the work performed in our laboratories, several examples of successful applications of differential display under a number of scenarios are reviewed. Differential display is one of several expression profiling technologies available and is compared with some of them. The future of differential display remains bright and is as applicable today as it was in 1992.

Affiliation(s): (4) Osteoporosis Research, Women’s Health Research Institute, Wyeth Research, Collegeville, PA
(5) Department of Medicine, Dartmouth Medical School, Hanover, NH
(6) Department of Medicine, Dartmouth Hitchcock Medical Center, Hanover, NH

Book Title: Cancer Genomics and Proteomics: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 383 | Year: 2007 | Page Range: 31-40 | DOI: 10.1007/978-1-59745-335-6_3

Subject: Cancer Research

Key Words: Differential display - gene expression - RT-PCR - pancreatic cancer - thioredoxin apoptosis

References

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