SpringerProtocols: Abstract: Reciprocal Subtraction Differential RNA Display (RSDD): An Efficient Technology for Cloning Differentially Expressed Genes

Reciprocal Subtraction Differential RNA Display (RSDD): An Efficient Technology for Cloning Differentially Expressed Genes

By: Devanand Sarkar⁴, Dong-chul Kang⁵, Paul B. Fisher^{4 6 7 8}

Abstract

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Identification of differentially expressed genes is an essential step in comprehending the molecular basis of complex physiological and pathological processes. Subtraction hybridization and differential RNA display (DDRT-PCR) are two methods that are widely and successfully employed to clone differentially expressed genes. Unfortunately, both methods have inherent problems and limitations requiring improvements in the technique. A combination of these two methods termed reciprocal subtraction differential RNA display is described here that considerably reduces the complexity of DDRT-PCR and facilitates the rapid and efficient identification and cloning of both abundant and rare differentially expressed genes.

Affiliation(s): (4) Department of Urology, Columbia University Medical Center, College of Physicians and Surgeons, New York, NY
(5) Ilsong Institute of Life Science, Hallym University, Anyang, Kyeonggi-do, Republic of Korea
(6) Herbert Irving Comprehensive Cancer Center, College of Physicians and Surgeons, Columbia University Medical Center, New York, NY
(7) Department of Pathology, College of Physicians and Surgeons, Columbia University Medical Center, New York, NY
(8) Department of Neurosurgery, College of Physicians and Surgeons, Columbia University Medical Center, New York, NY

Book Title: Cancer Genomics and Proteomics: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 383 | Year: 2007 | Page Range: 1-14 | DOI: 10.1007/978-1-59745-335-6_1

Subject: Cancer Research

Key Words: Cloning - differential RNA display - reciprocal subtraction differential RNA display - RSDD - subtraction hybridization - tumor progression - gene expression

References

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