SpringerProtocols: Abstract: Poststaining Grids for Transmission Electron Microscopy: Conventional and Alternative Protocols

Poststaining Grids for Transmission Electron Microscopy: Conventional and Alternative Protocols

By: E. Ann Ellis²

Abstract

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Poststaining ultrathin sections on grids is an essential process in preparing specimens for examination using transmission electron microscopy. This process commonly consists of staining in aqueous solutions of uranyl acetate followed by lead citrate and can result in good consistent quality staining if certain precautions are taken. This chapter covers conventional and alternative methods for poststaining ultrathin sections, including micro-wave-assisted procedures and corrective measures when problems are encountered.

Affiliation(s): (2) Microscopy and Imaging Center, Texas A & M University, College Station, TX

Book Title: Electron Microscopy: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 369 | Pub. Date: Feb-27-2007 | Page Range: 97-106 | DOI: 10.1007/978-1-59745-294-6_6

Subject: Biochemistry

Key Words: Poststaining - uranyl acetate - lead citrate - microwave staining - precipitates

References

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