SpringerProtocols: Abstract: Microwave-Assisted Processing and Embedding for Transmission Electron Microscopy

Microwave-Assisted Processing and Embedding for Transmission Electron Microscopy

By: Paul Webster²

Abstract

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Microwave processors can provide a means of rapid processing and resin embedding for biological specimens that are to be sectioned and examined by transmission electron microscopy. This chapter describes a microwave-assisted protocol for processing, dehydrating, and embedding biological material, from living specimens to blocks embedded in sectionable resin in 4 h or less.

Affiliation(s): (2) Ahmanson Advanced Electron Microscopy and Imaging Center, House Ear Institute, Los Angeles, CA

Book Title: Electron Microscopy: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 369 | Pub. Date: Feb-27-2007 | Page Range: 47-65 | DOI: 10.1007/978-1-59745-294-6_4

Subject: Biochemistry

Key Words: Transmission electron microscopy - resin embedding - rapid fixation - microwave processing

References

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