SpringerProtocols: Abstract: Cell-Free Extract Systems and the Cytoskeleton: Preparation of Biochemical Experiments for Transmission Electron Microscopy

Cell-Free Extract Systems and the Cytoskeleton: Preparation of Biochemical Experiments for Transmission Electron Microscopy

By: Margaret Coughlin², William M. Brieher², Ryoma Ohi²

Abstract

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Cell-free systems can be used to reconstitute complex actin or microtubule-based phenomena. For example, a number of extracts support actin-dependent propulsion of Listeria monocytogenes, whereas Xenopus laevis extracts support formation of a microtubule-based meiotic spindle. Working in vitro opens these complex processes to biochemical dissection. Here, we describe methods to view these in vitro preparations by thin-section electron microscopy.

Affiliation(s): (2) Department of Systems Biology, Harvard Medical School, Boston, MA

Book Title: Electron Microscopy: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 369 | Pub. Date: Feb-27-2007 | Page Range: 199-212 | DOI: 10.1007/978-1-59745-294-6_10

Subject: Biochemistry

Key Words: Listeria monocytogenes - Xenopus laevis - cell-free extract - actin - microtubule

References

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