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Synthesis and Assay of Recombinant Adenovirus Protease
Abstract
All adenoviruses (Ads) sequenced so far encode a single endopeptidase of the cysteine class, named adenain. The Ad2 adenain is a 204-residue, nearly inactive monomer, which is activated during virus maturation by an 11-residue cleavage fragment of capsid protein pVI. This chapter describes the synthesis, purification, activation, and assay of recombinant human Ad type adenain.
Affiliation(s): (2) Département de Microbiologie et d’Infectiologie, Université de Sherbrooke, Sherbrooke, Québec, Canada
Series: Methods in Molecular Medicine  |  Volume: 131  |  Pub. Date: Feb-26-2007  |  Page Range: 251-255  |  DOI: 10.1007/978-1-59745-277-9_18
Subject:  Microbiology
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