SpringerProtocols: Abstract: Computational Gene Annotation in New Genome Assemblies Using GeneID

12. Computational Gene Annotation in New Genome Assemblies Using GeneID

By: Enrique Blanco², Josep F. Abril²

Abstract

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The sequence of many eukaryotic genomes is nowadays available from a personal computer to any researcher in the world-wide scientific community. However, the sequences are worthless without the adequate annotation of the biological meaningful elements. The annotation of the genes, in particular, is a challenging task that can not be tackled without the aid of specific bioinformatics tools. We present in this chapter a simple protocol mainly based on the combination of the program GeneID and other computational tools to annotate the location of a gene, which was previously annotated in D. melanogaster, in the recently assembled genome of D. yakuba.

Affiliation(s): (2) Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, Barcelona, Spain

Book Title: Bioinformatics for DNA Sequence Analysis

Series: Methods in Molecular Biology | Volume: 537 | Pub. Date: Jan-01-2009 | Page Range: 243-261 | DOI: 10.1007/978-1-59745-251-9_12

Subject: Bioinformatics

Key Words: Gene finding - gene - protein - genome - exon - intron - sequence alignment - annotation pipeline

References

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