SpringerProtocols: Abstract: 1. Yeast Mating: A Model System for Studying Cell and Nuclear Fusion

1. Yeast Mating: A Model System for Studying Cell and Nuclear Fusion

By: Casey A. Ydenberg³, Mark D. Rose³

Abstract

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Haploid yeast cells mate to form a zygote, whose progeny are diploid cells. A fundamentally sexual event, related to fertilization, yeast mating nevertheless exhibits cytological properties that appear similar to somatic cell fusion. A large collection of mutations that lead to defects in various stages of mating, including cell fusion, has allowed a detailed dissection of the overall pathway. Recent advances in imaging methods, together with powerful methods of genetic analysis, make yeast mating a superb platform for investigation of cell fusion. An understanding of yeast cell fusion will provide insight into fundamental mechanisms of cell signaling, cell polarization, and membrane fusion.

Affiliation(s): (3) Department of Molecular Biology, Princeton University, Princeton, NJ

Book Title: Cell Fusion: Overviews and Methods

Series: Methods in Molecular Biology | Volume: 475 | Pub. Date: Jul-01-2008 | Page Range: 3-20 | DOI: 10.1007/978-1-59745-250-2_1

Subject: Cell Biology

Key Words: Conjugation - mating - Saccharomyces cerevisiae - pheromone - cell polarity - karyogamy

References

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