SpringerProtocols: Abstract: 6. Detection of Cytoplasmic and Nuclear Localization of Adenomatous Polyposis Coli (APC) Protein in Cells

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6. Detection of Cytoplasmic and Nuclear Localization of Adenomatous Polyposis Coli (APC) Protein in Cells

By: Mariana Brocardo³, Beric R. Henderson³

Abstract

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The adenomatous polyposis coli (APC) tumour suppressor gene is mutated in the majority of colon cancers. APC is a multi-domain protein whose distribution at different subcellular locations correlates with unique cellular processes. Our laboratory has focused on the link between APC subcellular location and function, and has characterized pathways for the trafficking of APC both into and out of the nucleus. Antibody specificity is an important factor in the determination of APC localization, and in this chapter we outline a strategy for the unambiguous detection of APC using a combination of biochemical and cell biology approaches.

Affiliation(s): (3) Westmead Millennium Institute, Westmead, NSW, Australia, Westmead, NSW, Australia

Book Title: Wnt Signaling: Pathway Methods and Mammalian Models

Series: Methods in Molecular Biology | Volume: 468 | Pub. Date: Jul-01-2008 | Page Range: 77-89 | DOI: 10.1007/978-1-59745-249-6_6

Subject: Protein Science

Key Words: APC - Western blotting - Subcellular localization - Colon cancer - Immunofluorescence microscopy - Antibodies - APC detection - Electrophoresis

References

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