SpringerProtocols: Abstract: 13. Measuring CamKII Activity in Xenopus Embryos as a Read-out for Non-canonical Wnt Signaling

13. Measuring CamKII Activity in Xenopus Embryos as a Read-out for Non-canonical Wnt Signaling

By: Michael Kühl³, Petra Pandur³

Abstract

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It has been known for quite some time that not all members of the Wnt family induce the formation of a secondary body axis when ectopically expressed in Xenopus embryos. An ingenious hypothesis led to the discovery that some Wnt ligands have the capacity to elicit intracellular Ca²⁺ signaling. This finding has been studied in more detail in the past years, which has revealed an intriguing complexity of Wnt signaling. The significance of a Wnt-induced Ca²⁺ -mediated pathway during development has been demonstrated in various model systems so far and includes processes such as dorsal—ventral patterning, regulation of the canonical Wnt/β-catenin signaling pathway, tumor formation, bone formation, and regulation of epithelial—mesenchymal transitions. Here we describe two assays to measure the activation of the Ca²⁺ /calmodulin-dependent kinase (CamK)-II, a Ca²⁺ -sensitive molecule described as a mediator of a non-canonical Wnt signaling pathway.

Affiliation(s): (3) University of Ulm, Institute for Biochemistry and Molecular Biology, Ulm, Germany

Book Title: Wnt Signaling: Pathway Methods and Mammalian Models

Series: Methods in Molecular Biology | Volume: 468 | Pub. Date: Jul-01-2008 | Page Range: 173-186 | DOI: 10.1007/978-1-59745-249-6_13

Subject: Protein Science

Key Words: Xenopus - Wnts - Non-canonical signaling - Calcium - Ca2+ /calmodulin-dependent kinase II - CamKII - Enzyme activity assay

References

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