Adipose tissue plays a critical role in energy homeostasis, not only in storing triglycerides, but also in responding to nutrient, neural, and hormonal signals, and secreting adipokines that control feeding, thermogenesis, immunity, and neuroendocrine function. It is conceivable that adipose tissue-specific gene expression would influence the aforementioned functions. A feasible approach to prepare adipose tissue-specific transgenic mouse models is necessary for such studies. Here, we report the preparation of adipose tissue-specific cholesteryl ester transfer protein transgenic mice. The general principle might apply to the establishment of other adipose tissue-specific transgenic mice models.