Springer Protocols: Abstract: 22. Determination of Lipolysis in Isolated Primary Adipocytes

22. Determination of Lipolysis in Isolated Primary Adipocytes

By: Srikant Viswanadha³, Constantine Londos³

Abstract

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Lipolysis involves the sequential breakdown of triglycerides into free fatty acids and glycerol. The extent of lipolysis is therefore a key determinant of the energy status of an individual and also dictates insulin resistance. Here, we describe a protocol for estimating lipolysis in murine adipocytes. Glycerol released during the lipolytic reaction is estimated radiometrically to determine the extent of lipolysis within the cell and the data are normalized to cell number.

Affiliation(s): (3) Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland

Book Title: Adipose Tissue Protocols

Series: Methods in Molecular Biology | Volume: 456 | Pub. Date: Jun-01-2008 | Page Range: 299-306 | DOI: 10.1007/978-1-59745-245-8_22

Subject: Molecular Medicine

Key Words: Triglycerides - fatty acids - glycerol

References

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