Springer Protocols: Abstract: 8. Methods for Conversion of Prion Protein into Amyloid Fibrils

8. Methods for Conversion of Prion Protein into Amyloid Fibrils

By: Leonid Breydo³, Natallia Makarava³, Ilia V. Baskakov³

Abstract

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Misfolding and aggregation of prion protein (PrP) is related to several neurodegenerative diseases in humans such as Creutzfeldt—Jacob disease, fatal familial insomnia, and Gerstmann—Straussler—Sheinker disease. Amyloid fibrils prepared from recombinant PrP in vitro share many features of the infectious prions. These fibrils can be used as a synthetic surrogate of PrP^Sc for development of prion diagnostics, including generation of PrP^Sc-specific antibody, for screening of antiprion drugs, or for development of antiprion decontamination procedures. Here, we describe the methods of preparation of prion protein fibrils in vitro and biochemical assays for assessing physical properties and the quality of fibrils.

Affiliation(s): (3) Medical Biotechnology Center, University of Maryland Biotechnology Institute, Baltimore, MD, USA

Book Title: Prion Protein Protocols

Series: Methods in Molecular Biology | Volume: 459 | Pub. Date: Jun-04-2008 | Page Range: 105-115 | DOI: 10.1007/978-1-59745-234-2_8

Subject: Protein Science

Key Words: Amyloid fibrils - conformational transition - prion diseases - recombinant prion protein

References

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