Springer Protocols: Abstract: 7. Understanding the Nature of Prion Diseases Using Cell-free Assays

7. Understanding the Nature of Prion Diseases Using Cell-free Assays

By: Victoria A. Lawson³

Abstract

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A central event in the transmission and pathogenesis of transmissible spongiform encephalopathy diseases is the misfolding of the prion protein. Considerable progress has been made in our understanding of this misfolding event through the development of cell-free assays that mimic the molecular features of prion propagation. This chapter reviews the contribution of cell-free assays to our understanding of prion propagation.

Affiliation(s): (3) Department of Pathology and Mental Health Research Institute of Victoria, University of Melbourne, Melbourne, Australia

Book Title: Prion Protein Protocols

Series: Methods in Molecular Biology | Volume: 459 | Pub. Date: Jun-04-2008 | Page Range: 97-103 | DOI: 10.1007/978-1-59745-234-2_7

Subject: Protein Science

Key Words: Cell-free conversion (CFC) - protein misfolding cyclic amplification assay (PMCA) - prion - recombinant

References

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