Springer Protocols: Abstract: 18. Quantitative Bioassay of Surface-bound Prion Infectivity

18. Quantitative Bioassay of Surface-bound Prion Infectivity

By: Victoria A. Lawson³

Abstract

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The unconventional nature of the infectious agent of prion diseases poses a challenge to conventional infection control methodologies. The extra neural tissue distribution of variant and sporadic Creutzfeldt—Jakob disease has increased concern regarding the risk of prion disease transmission via general surgical procedures and highlighted the need for decontamination procedures that can be incorporated into routine processing. This chapter describe a quantitative method for assessing the prionocidal activity of chemical and physical decontamination methods against surface-bound prion infectivity.

Affiliation(s): (3) Department of Pathology and Mental Health Research Institute of Victoria, University of Melbourne, Melbourne, Australia

Book Title: Prion Protein Protocols

Series: Methods in Molecular Biology | Volume: 459 | Pub. Date: Jun-04-2008 | Page Range: 265-273 | DOI: 10.1007/978-1-59745-234-2_18

Subject: Protein Science

Key Words: Bioassay - decontamination - prion - quantitative - steel wire

References

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