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10. Expression and Purification of Full-Length Recombinant PrP of High Purity
Abstract
Certain applications in the prion field require recombinant prion protein (PrP) of high purity and quality. Here, we report an experimental procedure for expression and purification of full-length mammalian prion protein. This protocol has been proved to yield PrP of extremely high purity that lacks PrP adducts, which are normally generated as a result of spontaneous oxidation or degradation.
Affiliation(s): (3) Medical Biotechnology Center, University of Maryland Biotechnology Institute, Baltimore, MD, USA
Series: Methods in Molecular Biology  |  Volume: 459  |  Pub. Date: Jun-04-2008  |  Page Range: 131-143  |  DOI: 10.1007/978-1-59745-234-2_10
Subject:  Protein Science
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