SpringerProtocols: Abstract: Improving Marginal Crystals

Improving Marginal Crystals

By: Charles W. Carter Jr.², Madeleine Riès-Kautt³

Abstract

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The physical chemistry of crystal growth can help to identify directions in which to look for improved crystal properties. In this chapter, we summarize how crystal growth depends on parameters that can be controlled experimentally, and relate them to the tools available for optimizing a particular crystal form for crystal shape, volume, and diffraction quality. Our purpose is to sketch the conceptual basis of optimization and to provide sample protocols derived from those foundations. We hope to assist even those who chose not to use systematic methods by enabling them to carry out rudimentary optimization searches armed with a better understanding of how the underlying physical chemistry operates.

Affiliation(s): (2) Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC
(3) Laboratoire de Cristallographie et RMN Biologiques, Université René Descartes, Paris, France

Book Title: Macromolecular Crystallography Protocols: Volume 1, Preparation and Crystallization of Macromolecules

Series: Methods in Molecular Biology | Volume: 363 | Pub. Date: Feb-05-2007 | Page Range: 153-174 | DOI: 10.1007/978-1-59745-209-0_8

Subject: Protein Science

Key Words: Protein crystallization - optimization - crystallogenesis - solubility - supersaturation - nucleation - crystal growth - Hofmeister series - anions - cations - salts - polymers - response surface - stationary points

References

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