SpringerProtocols: Abstract: Production of Selenomethionyl Proteins in Prokaryotic and Eukaryotic Expression Systems

Production of Selenomethionyl Proteins in Prokaryotic and Eukaryotic Expression Systems

By: Sylvie Doublié²

Abstract

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The use of selenomethionine as a phasing tool was first reported in 1990. Engineering of selenomethionyl proteins for structure determination is now routine. In fact, selenium is by far the most commonly used anomalous scatterer for multiwavelength anomalous diffraction studies. The past few years have seen new developments, which demonstrated the feasibility of expressing selenomethionyl protein in eukaryotic systems. In this chapter, the different methods available for producing selenomethionine-labeled proteins in bacteria, as well as in yeast and mammalian cells will be presented, along with tips for purifying and crystallizing selenomethionyl proteins.

Affiliation(s): (2) Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, VT

Book Title: Macromolecular Crystallography Protocols: Volume 1, Preparation and Crystallization of Macromolecules

Series: Methods in Molecular Biology | Volume: 363 | Pub. Date: Feb-05-2007 | Page Range: 91-108 | DOI: 10.1007/978-1-59745-209-0_5

Subject: Protein Science

Key Words: Selenomethionine - selenomethionyl protein expression - selenocysteine - multiwavelength anomalous diffraction

References

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