SpringerProtocols: Abstract: Human Hepatocyte Transplantation Overview

1. Human Hepatocyte Transplantation Overview

By: Juliana Puppi¹, Anil Dhawan²

Abstract

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The interest in hepatocyte transplantation has been growing continuously in recent years and this treatment may represent an alternative clinical approach for patients with acute liver failure and liver-based metabolic disorders. This chapter presents an overview of liver cell transplantation, from the basic research to human experience. It summarizes the pre-clinical studies and present status of clinical hepatocyte transplantation and identifies some possible areas of future research in this area.

Affiliation(s): (1) Institute of Liver Studies, King’s College London School of Medicine London, UK
(2) Paediatric Liver Centre, King’s College Hospital, Denmark Hill, London, UK

Book Title: Hepatocyte Transplantation: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 481 | Year: 2009 | Page Range: 1-16 | DOI: 10.1007/978-1-59745-201-4_1

Subject: Molecular Medicine

Key Words: Hepatocyte transplantation - collagenase - cryopreservation - sources of liver tissues - GMP laboratory - clinical experience - future use

References

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1.	Pereira, S. P., McCarthy, M., Ellis, A. J., et al. (1997) Auxiliary partial orthotopic liver transplantation for acute liver failure. J Hepatol 26, 1010–1017.

2.	Strom, S. C., Fisher, R. A., Thompson, M. T., et al. (1997) Hepatocyte transplantation as a bridge to orthotopic liver transplantation in terminal liver failure. Transplantation 63, 559–569.

3.	Bilir, B. M., Guinette, D., Karrer, F., et al. (2000) Hepatocyte transplantation in acute liver failure. Liver Transpl 6, 32–40.

4.	Schneider, A., Attaran, M., Meier, P. N., et al. (2006) Hepatocyte transplantation in an acute liver failure due to mushroom poisoning. Transplantation 82, 1115–1116.

5.	Fox, I. J., Chowdhury, J. R., Kaufman, S. S., et al. (1998) Treatment of the Crigler–Najjar syndrome type I with hepatocyte transplantation. N Engl J Med 338, 1422–1426.

6.	Ambrosino, G., Varotto, S., Strom, S. C., et al. (2005) Isolated hepatocyte transplantation for Crigler–Najjar syndrome type 1. Cell Transpl 14, 151–157.

7.	Muraca, M., Gerunda, G., Neri, D., et al. (2002) Hepatocyte transplantation as a treatment for glycogen storage disease type 1a. Lancet 359, 317–318.

8.	Horslen, S. P., McCowan, T. C., Goertzen, T. C., et al. (2003) Isolated hepatocyte transplantation in an infant with a severe urea cycle disorder. Pediatrics 111, 1262–1267.

9.	Stephenne, X., Najimi, M., Sibille, C., et al. (2006) Sustained engraftment and tissue enzyme activity after liver cell transplantation for argininosuccinate lyase deficiency. Gastroenterology 130, 1317–1323.

10.	Mitry, R. R., Hughes, R. D., Aw, M. M., et al. (2003) Human hepatocyte isolation and relationship of cell viability to early graft function. Cell Transpl 12, 69–74.

11.	Mitry, R. R., Dhawan, A., Hughes, R. D., et al. (2004) One liver, three recipients: segment IV from split-liver procedures as a source of hepatocytes for cell transplantation. Transplantation 77, 1614–1616.

12.	Muiesan, P. (2003) Can controlled non-heart-beating donors provide a solution to the organ shortage? Transplantation 75, 1627–1628.

13.	Hughes, R. D., Mitry, R. R., Dhawan, A., et al. (2006) Isolation of hepatocytes from livers from non-heart-beating donors for cell transplantation. Liver Transpl 12, 713–717.

14.	Kobayashi, N., Fujiwara, T., Westerman, K. A., et al. (2000) Prevention of acute liver failure in rats with reversibly immortalized human hepatocytes. Science 287, 1258–1262.

15.	Cai, J., Ito, M., Nagata, H., et al. (2002) Treatment of liver failure in rats with end-stage cirrhosis by transplantation of immortalized hepatocytes. Hepatology 36, 386–394.

16.	Dan, Y. Y., Riehle, K. J., Lazaro, C., et al. (2006) Isolation of multipotent progenitor cells from human fetal liver capable of differentiating into liver and mesenchymal lineages. Proc Natl Acad Sci USA 103, 9912–9917.

17.	Avital, I., Feraresso, C., Aoki, T., et al. (2002) Bone marrow-derived liver stem cell and mature hepatocyte engraftment in livers undergoing rejection. Surgery 132, 384–390.

18.	Miki, T., Lehmann, T., Cai, H., et al. (2005) Stem cell characteristics of amniotic epithelial cells. Stem Cells 23, 1549–1559.

19.	Ruhnke, M., Ungefroren, H., Nussler, A., et al. (2005) Differentiation of in vitro-modified human peripheral blood monocytes into hepatocyte-like and pancreatic islet-like cells. Gastroenterology 128, 1774–1786.

20.	Strom, S. C., Dorko, K., Thompson, M. T., et al. (1998) Large scale isolation and culture of human hepatocytes, in (Franco, D., et al. ed.), ýlots de Langerhans et hýpatocytes: vers une utilisation therapeutique, pp. 195–205. Les Editions INSERM, Paris.

21.	Terry, C., Dhawan, A., Mitry, R. R., et al. (2006) Cryopreservation of isolated human hepatocytes for transplantation: State of the art. Cryobiology 53, 149–159.

22.	Diener, B., Utesch, D., Beer, N., et al. (1993) A method for the cryopreservation of liver parenchymal cells for studies of xenobiotics. Cryobiology 30, 116–127.

23.	Horslen, S. P., Fox, I. J. (2004) Hepatocyte transplantation. Transplantation 77, 1481–1486.

24.	Fox, I. J., Roy-Chowdhury, J. (2004) Hepatocyte transplantation. J Hepatol 40, 878–886.

25.	Krishna Vanaja, D., Sivakumar, B., Jesudasan, R. A., et al. (1998) In vivo identification, survival, and functional efficacy of transplanted hepatocytes in acute liver failure mice model by FISH using Y-chromosome probe. Cell Transpl 7, 267–273.

26.	Sutherland, D. E., Numata, M., Matas, A. J., et al. (1977) Hepatocellular transplantation in acute liver failure. Surgery 82, 124–132.

27.	Baumgartner, D., LaPlante-O'Neill, P. M., Sutherland, D. E., et al. (1983) Effects of intrasplenic injection of hepatocytes, hepatocyte fragments and hepatocyte culture supernatants on D-galactosamine-induced liver failure in rats. Eur Surg Res 15, 129–135.

28.	Demetriou, A. A., Reisner, A., Sanchez, J., et al. (1988) Transplantation of microcarrier-attached hepatocytes into 90% partially hepatectomized rats. Hepatology 8, 1006–1009.

29.	Rozga, J., Holzman, M., Moscioni, A. D., et al. (1995) Repeated intraportal hepatocyte transplantation in analbuminemic rats. Cell Transpl 4, 237–243.

30.	De Vree, J. M., Ottenhoff, R., Bosma, P. J., et al. (2000) Correction of liver disease by hepatocyte transplantation in a mouse model of progressive familial intrahepatic cholestasis. Gastroenterology 119, 1720–1730.

31.	Laconi, E., Oren, R., Mukhopadhyay, D. K., et al. (1998) Long-term, near-total liver replacement by transplantation of isolated hepatocytes in rats treated with retrorsine. Am J Pathol 153, 319–329.

32.	Guha, C., Parashar, B., Deb, N. J., et al. (2002) Normal hepatocytes correct serum bilirubin after repopulation of Gunn rat liver subjected to irradiation/partial resection. Hepatology 36, 354–362.

33.	Strom, S. C., Chowdhury, J. R., Fox, I. J. (1999) Hepatocyte transplantation for the treatment of human disease. Semin Liver Dis 19, 39–48.

34.	Grossman, M., Raper, S. E., Kozarsky, K., et al. (1994) Successful ex vivo gene therapy directed to liver in a patient with familial hypercholesterolaemia. Nat Genet 6, 335–341.

35.	Grossman, M., Rader, D. J., Muller, D. W., et al. (1995) A pilot study of ex vivo gene therapy for homozygous familial hypercholesterolaemia. Nat Med 1, 1148–1154.

36.	Strom, S. C., Fisher, R. A., Rubinstein, W. S., et al. (1997) Transplantation of human hepatocytes. Transpl Proc 29, 2103–2106.

37.	Stephenne, X., Najimi, M., Smets, F., et al. (2005) Cryopreserved liver cell transplantation controls ornithine transcarbamylase deficient patient while awaiting liver transplantation. Am J Transpl 5, 2058–2061.

38.	Sokal, E. M., Smets, F., Bourgois, A., et al. (2003) Hepatocyte transplantation in a 4-year-old girl with peroxisomal biogenesis disease: technique, safety, and metabolic follow-up. Transplantation 76, 735–738.

39.	Dhawan, A., Mitry, R. R., Hughes, R. D., et al. (2004) Hepatocyte transplantation for inherited factor VII deficiency. Transplantation 78, 1812–1814.

40.	Thompson, R., Strautnieks, S. (2001) BSEP: function and role in progressive familial intrahepatic cholestasis. Semin Liver Dis 21, 545–550.

41.	Aronsen, K. F., Nylander, G. (1967) Use of direct protography in diagnosis of liver diseases. Radiology 88, 40–47.

42.	Goss, J. A., Soltes, G., Goodpastor, S. E., et al. (2003) Pancreatic islet transplantation: the radiographic approach. Transplantation 76, 199–203.

43.	Maleux, G., Gillard, P., Keymeulen, B., et al. (2005) Feasibility, safety, and efficacy of percutaneous transhepatic injection of beta-cell grafts. J Vasc Interv Radiol 16, 1693–1697.

44.	Owen, R. J., Ryan, E. A., O'Kelly, K., et al. (2003) Percutaneous transhepatic pancreatic islet cell transplantation in type 1 diabetes mellitus: radiologic aspects. Radiology 229, 165–170.

45.	Darwish, A. A., Sokal, E., Stephenne, X., et al. (2004) Permanent access to the portal system for cellular transplantation using an implantable port device. Liver Transpl 10, 1213–1215.

46.	Fox, I. J., Chowdhury, J. R. (2004) Hepatocyte transplantation. Am J Transpl 4 Suppl 6, 7–13.

47.	Terry, C., Dhawan, A., Mitry, R. R., et al. (2005) Preincubation of rat and human hepatocytes with cytoprotectants prior to cryopreservation can improve viability and function upon thawing. Liver Transpl 11, 1533–1540.

48.	Mas, V. R., Maluf, D. G., Thompson, M., et al. (2004) Engraftment measurement in human liver tissue after liver cell transplantation by short tandem repeats analysis. Cell Transpl 13, 231–236.

49.	Wang, L. J., Chen, Y. M., George, D., et al. (2002) Engraftment assessment in human and mouse liver tissue after sex-mismatched liver cell transplantation by real-time quantitative PCR for Y chromosome sequences. Liver Transpl 8, 822–828.

50.	Rogers, W. J., Meyer, C. H., Kramer, C. M. (2006) Technology insight: in vivo cell tracking by use of MRI. Nat Clin Pract Cardiovasc Med 3, 554–562.

51.	Soriano, H. E., Wood, R. P., Kang, D. C. (1997) Hepatocellular transplantation in children with fulminant liver failure. Hepatology 30, 239A.

52.	Sterling, R. K., Fisher, R. A. (2001) Liver transplantation: Living donor, hepatocyte, and xenotransplantation, in (Gish, R., ed.), Current Future Treatment Therapies for Liver Disease. Clinics in Liver Disease, WB Saunders, Philadelphia.

53.	Habibullah, C. M., Syed, I. H., Qamar, A., et al. (1994) Human fetal hepatocyte transplantation in patients with fulminant hepatic failure. Transplantation 58, 951–952.

54.	Fisher, R. A., Strom, S. C. (2000) Human hepatocyte transplantation: Biology and therapy, in (Berry, M. N., Edwards, A. M., ed.), Hepatocyte Review, Kluwer Academic Publishers, Dordrecht, The Netherlands.

55.	Fisher, R. A., Bu, D., Thompson, M., et al. (2000) Defining hepatocellular chimerism in a liver failure patient bridged with hepatocyte infusion. Transplantation 69, 303–307.

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