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205. Screening Hybridoma Culture Supernatants Using ELISA
Enzyme-linked immunosorbent assay (ELISA) is a widely used method for the detection of antibody and is appropriate for use for screening hybridoma superna-tants (1). As with radiobinding assays, it is a solid-phase binding assay that is quick, reliable, and accurate. The method is often preferred to radioactive assays, since the handling and disposal of radioisotopes is avoided and the enzyme conjugates are more stable than radioiodinated proteins. However, most of the substrates for the enzyme reactions are carcinogenic or toxic and, hence, require handling with care.
Affiliation(s): (1) Apovia, Inc., CA, San Diego, USA
(2) Division of Immunobiology, National Institute for Biological Standards and Control, UK, Potters Bar, USA
Series: Springer Protocols Handbooks  |  Year: 2009  |  Page Range: 1947-1948  |  DOI: 10.1007/978-1-59745-198-7_205
Subject:  Protein Science
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