SpringerProtocols: Abstract: Analysis of Reconstituted Chromatin Using a Solid-Phase Approach

2. Analysis of Reconstituted Chromatin Using a Solid-Phase Approach

By: Raphael Sandaltzopoulos¹, Peter B. Becker²

Abstract

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Complex experimental strategies involving in vitro reconstituted chromatin or simple chromatin interaction studies are much facilitated by immobilizing the nucleosomal arrays to paramagnetic beads. Chromatin-containing beads can be retrieved from a reaction mix solution on a magnet fast and quantitatively, effectively separating bound, loosely attached and unbound components efficiently. This chapter details a convenient strategy for immobilization of linear plasmid DNA on streptavidin-coated beads, the reconstitution of chromatin on such beads and some fundamental handling procedures.

Affiliation(s): (1) Department of Molecular Biology and Genetics, Democritus University of Thrace, University Campus, Alexandroupolis, Greece
(2) Molekularbiologie, Adolf-Butenandt-Institut, München, Germany

Book Title: Chromatin Protocols: Second Edition

Series: Methods in Molecular Biology | Volume: 523 | Pub. Date: Dec-01-2008 | Page Range: 11-25 | DOI: 10.1007/978-1-59745-190-1_2

Subject: Cell Biology

Key Words: Biotin-streptavidin - Dynabeads - solid phase - chromatin assembly - chromatin constituents

References

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