Springer Protocols: Abstract: Scanning for DNA Variants by Denaturant Capillary Electrophoresis

Scanning for DNA Variants by Denaturant Capillary Electrophoresis

By: Per O. Ekstrøm⁴

Abstract

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Analysis and detection of DNA variation is important in any field of biology. Hence, numerous methods have been developed to analyze DNA. A simple yet effective way of analyzing DNA is by denaturant capillary electrophoresis (DCE). The method is in theory applicable to 95% of the human genome. The method involves three steps; fragment design, PCR amplification and allele separation. The allele separation can in principle be performed with any DNA capillary sequencing instrument.

Affiliation(s): (4) The Norwegian Radium Hospital, Oslo, Norway

Book Title: Genomics Protocols

Series: Methods in Molecular Biology | Volume: 439 | Pub. Date: Feb-01-2008 | Page Range: 19-34 | DOI: 10.1007/978-1-59745-188-8_2

Subject: Genetics/Genomics

Key Words: DGGE (denaturant gradient gel electrophoresis) - CDCE (constant denaturant capillary electrophoresis) - CTCE (cycling temperature capillary electrophoresis) - PCR (polymerase chain reaction) - dsDNA — double strand DNA - ssDNA — single strand DNA

References

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