Springer Protocols: Abstract: Isolation and Functional Characterization of Side Population Stem Cells

Isolation and Functional Characterization of Side Population Stem Cells

By: Jonathan B. Johnnidis³, Fernando D. Camargo³

Abstract

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The “side population” (SP) phenotype is a manifestation of primitive cells’ ability to efficiently efflux the fluorescent DNA-staining dye Hoechst 33342 and can be used as the basis by which to isolate these cells using flow cytometry. In the bone marrow (BM), the SP defines a cell subset with a highly homogeneous content of hematopoietic stem cells (HSCs). In this chapter, we describe a protocol to reproducibly isolate murine BM SP cells, as well as analytic measures, such as single cell transplantation, that can be used to assess the functionality of SP-derived stem cells.

Affiliation(s): (3) Whitehead Institute for Biomedical Research, Cambridge, MA

Book Title: Hematopoietic Stem Cell Protocols

Series: Methods in Molecular Biology | Volume: 430 | Pub. Date: Jan-01-2008 | Page Range: 183-193 | DOI: 10.1007/978-1-59745-182-6_13

Subject: Cell Biology

Key Words: Side population - Hoechst 33342 - stem cell - phenotype - dye efflux - purification

References

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