Springer Protocols: Abstract: Hematopoietic Stem Cell Transplant in Mice by Intra-Femoral Injection

Hematopoietic Stem Cell Transplant in Mice by Intra-Femoral Injection

By: Yuxia Zhan³, Yi Zhao³

Abstract

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In traditional bone marrow transplantation, the majority of peripherally introduced stem cells are trapped in peripheral organs, such as the lung and liver. The frequency of cells homed in bone marrow by such method is extremely low. This circumstance adds difficulty to the research of hematopoietic stem cell (HSC), a rare population to begin with. By introducing HSC directly into bone marrow cavity, the peripheral loss of HSC can be minimized. Thus, intra-femoral injection of HSC is a useful method for HSC study.

Affiliation(s): (3) Gene Therapy Laboratories, Norris Cancer Center, University of Southern California School of Medicine, Los Angeles, CA

Book Title: Hematopoietic Stem Cell Protocols

Series: Methods in Molecular Biology | Volume: 430 | Pub. Date: Jan-01-2008 | Page Range: 161-169 | DOI: 10.1007/978-1-59745-182-6_11

Subject: Cell Biology

Key Words: Hematopoietic stem cell (HSC) - bone marrow transplantation (BMT) - intra-femoral injection

References

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