SpringerProtocols: Abstract: Detection of SARS Coronavirus in Humans and Animals by Conventional and Quantitative (Real Time) Reverse Transcription Polymerase Chain Reactions

6. Detection of SARS Coronavirus in Humans and Animals by Conventional and Quantitative (Real Time) Reverse Transcription Polymerase Chain Reactions

By: J.S. Malik Peiris¹, Leo L.M. Poon¹

Abstract

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Severe acute respiratory syndrome is a novel human disease caused by a coronavirus of animal origin. Soon after the discovery SARS-CoV, several molecular assays were described for the detection of this virus. Of these, conventional and quantitative RT-PCR approaches were the primary tools for SARS-CoV RNA detection. In this chapter we describe a two-step conventional RT-PCR and a one-step quantitative RT-PCR that were used routinely in our laboratories during the SARS outbreak.

Affiliation(s): (1) Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, SAR

Book Title: SARS- and Other Coronaviruses: Laboratory Protocols

Series: Methods in Molecular Biology | Volume: 454 | Year: 2008 | Page Range: 1-12 | DOI: 10.1007/978-1-59745-181-9_6

Subject: Infectious Diseases

Key Words: SARS coronavirus - RT-PCR - Molecular detection - clinical diagnosis

References

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