SpringerProtocols: Abstract: 2. Fine Structure of the Autophagosome

2. Fine Structure of the Autophagosome

By: Eeva-Liisa Eskelinen³

Abstract

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This chapter describes the electron microscopic fine structure of early and late autophagic vacuoles in mammalian cells. Detailed instructions are given for the preparation of cells for conventional electron microscopy and for the identification of autophagic vacuoles by morphology. Electron microscopy remains one of the most accurate methods for quantitation of autophagic vacuole accumulation. Therefore, quantitation of autophagic vacuoles by electron microscopy and point counting is also described. Finally, a short description is given for preparation of ultra thin cryosections for immunogold labeling of autophagic vacuoles.

Affiliation(s): (3) Department of Biological and Environmental Sciences, Division of Biochemistry, University of Helsinki, Helsinki, Finland

Book Title: Autophagosome and Phagosome

Series: Methods in Molecular Biology | Volume: 445 | Pub. Date: May-01-2008 | Page Range: 11-28 | DOI: 10.1007/978-1-59745-157-4_2

Subject: Cell Biology

Key Words: Autophagy - electron microscopy - point counting - volume fraction - cryosectioning - immunoelectron microscopy

References

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