SpringerProtocols: Abstract: Optimization of Procedures for Cloning by Somatic Cell Nuclear Transfer in Mice

Optimization of Procedures for Cloning by Somatic Cell Nuclear Transfer in Mice

By: Young Gie Chung⁴, Shaorong Gao⁵, Keith E. Latham⁶

Abstract

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Cloning by somatic cell nuclear transfer is a complex procedure that is dependent on correct interactions between oocyte and donor cell genome. These interactions require minimal insult to either the oocyte or the transplanted nucleus. Available data also indicate that reprogramming the donor cell genome may be slow, so that the cloned embryo expresses genes typical of the donor cell, and thus has different characteristics from normal embryos. Procedures that minimize damage to the donor genome and that address the unique characteristics of the cloned construct should enhance the efficacy of the method.

Affiliation(s): (4) Advanced Cell Technology, Worcester, MA
(5) Department of Animal Sciences, University of Connecticut, Storrs, CT
(6) The Fels Institute, Temple University School of Medicine, Philadelphia, PA

Book Title: Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis

Series: Methods in Molecular Biology | Volume: 348 | Pub. Date: Jul-18-2006 | Page Range: 111-123 | DOI: 10.1007/978-1-59745-154-3_7

Subject: Cell Biology

Key Words: Cloning - nuclear transfer - culture medium

References

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