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10. Analysis of Geminivirus DNA Replication by 2-D Gel
Abstract
The technique described was developed for the separation of begomovirus DNA. DNA products resulting from and during geminiviral replication are characterized by the application of strand-specific separation and identification by strand-specific DNA probing of Southern blots. The mapping of the initiation site of complementary-strand DNA synthesis, by this technique is also presented.
Affiliation(s): (6) Biological Chemistry, John Innes Centre, Norwich, NR4 7UH, UK
Series: Methods in Molecular Biology  |  Volume: 451  |  Pub. Date: Apr-01-2008  |  Page Range: 135-143  |  DOI: 10.1007/978-1-59745-102-4_10
Subject:  Plant Sciences
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