SpringerProtocols: Abstract: Encapsulation of Enzymes Using Polymers and Sol-Gel Techniques

Encapsulation of Enzymes Using Polymers and Sol-Gel Techniques

By: Mónica Campás², Jean-Louis Marty³

Abstract

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This chapter describes two enzyme immobilization methods based on the biomolecule encapsulation into polymer matrices: the sol-gel technology and the entrapment into the polymer poly(vinyl alcohol) with styrylpyridinium groups (PVA-SbQ). The sol-gel technology is based on the formation of silica matrices of metal or semi-metal oxides through the aqueous processing of hydrolytically labile precursors. The encapsulation into PVA-SbQ involves the photo-cross-linking of the styrylpyridinium groups in order to create the polymer matrix. These networks are chemically stable and do not restrict the enzyme activ-ity. Both bioencapsulation strategies provide simple, easy, and low-cost methods for enzyme immobilization. They are versatile, as matrixes can be tailor-designed and used to entrap a large number of biomolecules. They present numerous applications, including the development of biooptical devices, biosensors and biocatalysts.

Affiliation(s): (2) BIOMEM, Centre de Phytopharmacie, Universit&#X00E9; de Perpignan, Perpignan Cedex, France
(3) BIOMEM, Centre de Phytopharmacie, Universit&#X00E9; de Perpignan, Perpignan Cedex, France

Book Title: Immobilization of Enzymes and Cells

Series: Methods in Biotechnology | Volume: 22 | Pub. Date: Mar-15-2006 | Page Range: 77-85 | DOI: 10.1007/978-1-59745-053-9_7

Subject: Biotechnology

Key Words: Entrapment - sol-gel technology - tetramethoxysilane - TMOS - methyltrimethoxysilane - MTMOS - poly(vinyl alcohol) bearing styrylpyridinium groups - PVA-SbQ - photo-cross-linking - polymer matrix - screen-printed graphite electrode

References

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