SpringerProtocols: Abstract: Imaging Ca2+ Signals in Xenopus Oocytes

Imaging Ca2+ Signals in Xenopus Oocytes

By: Sheila L. Dargan², Angelo Demuro², Ian Parker³

Abstract

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Xenopus oocytes have become a favored preparation in which to study the spatiotemporal dynamics of intracellular Ca²⁺ signaling. Advantages of the oocyte as a model cell system include its large size, lack of intracellular Ca²⁺ release channels other than the type 1 inositol trisphosphate receptor, and ease of expression of foreign receptors and channels. We describe the use of high-resolution fluorescence imaging techniques to visualize Ca²⁺ signals in Xenopus oocytes at levels ranging from global Ca²⁺ waves to single-channel Ca²⁺ microdomains.

Affiliation(s): (2) Department of Neurobiology and Behavior, McGaugh Hall, University of California Irvine, Irvine, CA
(3) Department of Neurobiology and Behavior, University of California Irvine, Irvine, CA

Book Title: Xenopus Protocols: Cell Biology and Signal Transduction

Series: Methods in Molecular Biology | Volume: 322 | Pub. Date: Dec-13-2005 | Page Range: 103-119 | DOI: 10.1007/978-1-59745-000-3_8

Subject: Cell Biology

Key Words: Ca2+ - caged IP3 - calcium - confocal - dye - flash photolysis - fluorescence - imaging - inositol - IP3 - linescan - microinjection - microscopy - receptor - signaling - TIR - video rate - Xenopus oocytes

References

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