SpringerProtocols: Abstract: Chromatin Assembly of DNA Templates Microinjected Into Xenopus Oocytes

Chromatin Assembly of DNA Templates Microinjected Into Xenopus Oocytes

By: Danièle Roche², Geneviève Almouzni², Jean-Pierre Quivy²

Abstract

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The packaging of deoxyribonucleic acid (DNA) into chromatin within the eukaryotic nucleus can affect processes such as DNA replication, transcription, recombination, and repair. Therefore, studies aimed at understanding at the molecular level how these processes are operating have to take into account the chromatin context. We present a method to assemble DNA into chromatin by nuclear microinjection into Xenopus oocytes. This method allows in vivo chromatin formation in a nuclear environment. We provide the experimental procedures for oocyte preparation, DNA injection, and analysis of the assembled chromatin.

Affiliation(s): (2) Research Section, Institut Curie, UMR218 du Centre National de la Recherche Scientifique (CNRS), Paris, France

Book Title: Xenopus Protocols: Cell Biology and Signal Transduction

Series: Methods in Molecular Biology | Volume: 322 | Pub. Date: Dec-13-2005 | Page Range: 139-147 | DOI: 10.1007/978-1-59745-000-3_10

Subject: Cell Biology

Key Words: Microinjection - oocytes - chromatin assembly - nucleosomes

References

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