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Preparation of Cell Suspensions for Transplantation
Abstract
Implantation of embryonic neural tissue into the central nervous system (CNS) can be either in the form of solid pieces or as a dissociated cell suspension; The latter approach has several advantages as the preferred mode of transplantation into the CNS: The tissue can be placed directly into any site within the brain (including deep subcortical sites not accessible for solid grafting), without the need for any additional steps to ensure a special vascularization of the transplants (e.g., previous cavitation); multiple grafts can be placed within any individual animal/patient; the co-grafting of several tissue types is more easily realized using this technique; and suspension grafts enable an assessment to be made of the number and viability of cells being implanted.
Affiliation(s): (5) Department of Neurology and the Centre for Brain Repair, University of Cambridge, Forvie Site, Cambridge, UK
(6) Department of Experimental Psychology and the Centre for Brain Repair, University of Cambridge, Cambridge, UK
(7) School of Biosciences, Cardiff University, Wales, UK
Series: Neuromethods  |  Volume: 36  |  Pub. Date: Oct-20-1999  |  Page Range: 195-205  |  DOI: 10.1007/978-1-59259-690-4_11
Subject:  Neuroscience
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