Raising Polyclonal Antibodies Using Nitrocellulose-Bound Antigen
Highly specific antibodies directed against minor proteins, present in small amounts in biological fluids, or against nonsoluble
cytoplasmic or membranous proteins, are often difficult to obtain. The main reasons for this are the small amounts of protein
available after the various classical purification processes and the low purity of the proteins.
Affiliation(s): (2) Laboratoire de Genettque, CNRS, Marseille, France Series: Methods in Molecular Biology | Volume: 80 | Pub. Date: Apr-02-1998 | Page Range: 5-13 | DOI: 10.1007/978-1-59259-257-9_2
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